Deletion Mutagenesis Identifies a Haploinsufficient Role for g-Zein in opaque2 Endosperm Modification1[C][W][OPEN]
نویسندگان
چکیده
Center for Plant Science Innovation, Beadle Center for Biotechnology (L.Y., Y.D., C.Z., D.R.H.), Department of Agronomy and Horticulture (L.Y., D.R.H.), and School of Biological Sciences (Y.D., C.Z.), University of Nebraska, Lincoln, Nebraska 68588; and United States Department of Agriculture-Agricultural Research Service Cereal Disease Laboratory, University of Minnesota, St. Paul, Minnesota 55108 (S.F.K.)
منابع مشابه
Functional Genomics of Maize Endosperm Maturation and Protein Quality
Quality Protein Maize (QPM) is a hard kernel variant of the high-lysine mutant, opaque2. Using γ-irradiation, we created opaque QPM variants to identify opaque-2 modifier genes and to investigate deletion mutagenesis combined with Illumina sequencing as a maize functional genomics tool. A K0326Y-QPM deletion mutant (line 107) was null for the 27and 50-kD γ-zeins and abolished vitreous endosperm...
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The opaque2 (o2) modifier genes convert the soft endosperm of an o2 mutant to a hard, vitreous phenotype. The primary biochemical change associated with the expression of these genes is a two- to threefold increase in synthesis of the 27-kD gamma-zein storage protein. To investigate the mechanism of modifier gene activity, we examined the level of gamma-zein mRNA and protein synthesis during th...
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Association genetics is a powerful method to track gene polymorphisms responsible for phenotypic variation, since it takes advantage of existing collections and historical recombination to study the correlation between large genetic diversity and phenotypic variation. We used a collection of 375 maize (Zea mays ssp. mays) inbred lines representative of tropical, American, and European diversity...
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Waxy maize is prevalently grown in China and other countries due to the excellent characters and economic value. However, its low content of lysine can't meet the nutritional requirements of humans and livestock. In the present study, we introgressed the opaque2 (o2) allele into waxy maize line Zhao OP-6/O2O2 by using marker-assisted selection (MAS) technique and successfully improved the lysin...
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